The two winners, Brønshøj BK and FC Hjørring were promoted to the 2010–11 Danish 1st Division, along with the runner-up from the West-division Hobro IK, after they beat their Eastern counterpart B.93 5–1 on aggregate in a two-legged promotion play-off.
This relegation was later annulled, as 1st Division relegatee BK Frem went bankrupt, forcing a further two-level-relegation to the Copenhagen Series and leaving an extra place open for Otterup.
On 6 March 2010 it was decided that the eight Superliga clubs' second teams should be withdrawn from the Second Division at the end of the season and be transferred to a separate reserves competition.
Therefore, the relegation to the Denmark Series was reduced from six teams to one, and the promotion the other way increased from six to nine.
The relegation of Otterup B&I for losing this playoff was later cancelled, as BK Frem went bankrupt and left another place open in the 2nd Division.