The 2009–10 Division 1 Féminine was the 36th edition of the women's league since its re-establishment by the French Football Federation.
The league began on 27 September 2009 and ended on 13 June 2010.
The championship was assured following the club's 5–0 victory over Montigny-le-Bretonneux on the final match day of the season.
Both Lyon and Juvisy will appear in next year's UEFA Women's Champions League.
The winner was determined at the annual UNFP Awards, which was held on 9 May.