This is a list of division winners and playoff matches in the regionally organized Eccellenza 2009–2010, which is the 6th level of Italian football.
Capriatese(as finalist of Coppa Italia Dilettanti) 1.^ Montesilvano deducted one point.
Bagnolese promoted to the Serie D while Virtus Pavullese qualified to the National play-off.
Stuoie Baracca Lugo and Comacchio Lidi relegated to the Promozione.
Fidene promoted to the Serie D while Anziolavinio qualified to the National play-off.