The number of team entries for this season was initially 265, but this was reduced to 264 when Newcastle Blue Star withdrew.
This round is the first in which Conference Premier teams join those from lower reaches of the National League System.
Since King's Lynn folded, Vauxhall Motors received a walkover to the second round.
This match, however, did not go ahead because of a frozen pitch and neither did the third attempt at playing this fixture on 22 December.
However, on 25 January, Maidstone were re-instated in the competition after Histon were found to have fielded an ineligible player.