It started in October 2009 and ended on 22 May 2010 with the final at Stade de France in which Toulouse defeated Biarritz 21–19 to win the trophy for the fourth time.

On 29 May 2009, Newport Gwent Dragons defeated Calvisano 42–17 in a play-off to secure the 24th and final qualification place.

[4] The brackets show each team's European Rugby Club Ranking at the start of the 2009–10 season.

The winner of the topmost quarter-final on each side of the bracket receives home-country advantage in its semi-final.

The club also enjoys large support in the Basque Country of Spain, a region with which it shares a cultural affinity.)