Belenenses were ending a ten-year stretch in the Portuguese top-level league, while Trofense returned to the Liga da Honra after just one year in the top flight.

However, Estrela de Amadora, who finished in 11th place, were instead relegated to the Liga Vitalis for economic problems.

Due to those relegations, Boavista and Gondomar, the 15th and 16th teams, respectively, should have remained in the Liga de Honra but both were still relegated due to economic difficulties for Boavista and involvement in the Apito Dourado case for Gondomar.

To make up for the loss of four teams (instead of the regular two), Penafiel and Carregado, losers of the previous year's Second Division promotion play-offs, were invited to the Liga de Honra.

Updated to games played on 2 May 2010Source: Liga Vitalis – Match Statistics (in Portuguese)