It began on 2 September 2009 with Round 1 and ended on 30 May 2010 with the Final held at a neutral venue.
F91 Dudelange were the defending champions, having won their fourth cup title last season.
Thirty-eight of them competed in matches, with the other thirteen teams were awarded a bye.
Bye: Blo Weiss Itzig, US Boevange, Claravallis Clervaux, FC Kopstal, Luna Oberkorn, AS Luxembourg-Porto, Olympia Christnach, Orania Vianden, FC Pratzerthal/Rédange, US Reisdorf, AS Remich, FC Schifflange 95, Yellow Boys Weiler The winners of Round 1 and those teams that got a bye competed in this round.
Twenty-two winners of Round 3 competed in this round, as well as fourteen teams from the Division of Honour (II), which entered the competition in this round.