The members of the MISL's second season teams were the Baltimore Blast, the Milwaukee Wave, the Monterrey La Raza, the Philadelphia KiXX, and the Rockford Rampage.
The season kicked off on November 13, 2009, with the NISL champion, Baltimore Blast, welcoming the Rockford Rampage.
The regular season concluded on March 21, 2010, with the Rockford Rampage hosting Monterrey La Raza.
This year also marks the first season since the KiXX founding that they would play at a new arena, the Liacouras Center, on the campus of Temple University.
Blue indicates bye into the MISL Championship Green indicates playoff berth clinched GP = Games Played, G = Goals, A = Assists, Pts = Points The format for the playoffs is the same as the 2008–09 NISL format.