The 2009–10 Penn State Lady Lions basketball team represented Pennsylvania State University in the 2009–10 NCAA Division I women's basketball season.

Mia Nickson sat out the season due to NCAA transfer rules.

[4] Tyra Grant and Julia Trogele are the only starters from the previous season that returned.

The Nittany Lions competed in the Maine Classic from November 27–28.

The WBCA Pink Zone at Penn State was held on January 24.