The 2009–10 Quaid-e-Azam Trophy was one of two first-class domestic cricket competitions that were held in Pakistan during the 2009–10 season.

It was the 52nd edition of the Quaid-e-Azam Trophy, contested by 22 teams representing regional cricket associations and departments,[a] and was followed in the schedule by the Pentangular Cup, contested by the top two regions and departments in the Quaid-e-Azam Trophy and a Rest of Pakistan team.

[4] In a change from 2007–08, the bottom two departmental teams were relegated to the Patron's Trophy (Grade II) competition for the following season.

[1][2] Karachi Blues beat Habib Bank Limited by 141 runs in the final to win the trophy.

In a low scoring match, Habib Bank, chasing a victory target of 208, were bowled out for just 66 runs in the fourth innings.