The draw for the First Round was made at Hamilton Crescent, popularly known as the West of Scotland Cricket Ground, at 15:00 on 3 September 2009.
[3][4] Hawick Royal Albert's defeat by Huntly led to a police investigation of an allegation that the match had been fixed.
[7] Source: BBC Sport Archived 18 January 2009 at the Wayback Machine Source: BBC Sport Archived 18 January 2009 at the Wayback Machine The 16 winners from the Third Round entered here, along with the 12 SPL clubs and four SFL First Division clubs who were exempt from playing in the Third Round.
As well as this, the club's management failed to register the two mandatory under-21 players required by the rules and submitted an inaccurate team sheet.
As a result of these breaches in the rules, Dunfermline Athletic were to be expelled from the competition and Stenhousemuir were to progress their stead.