The 2009–10 Slovak Cup was the 41st season of Slovakia's annual knock-out cup competition and the seventeenth since the independence of Slovakia.
It began on 5 August 2009 with the matches of first round and ended on 11 May 2010 with the final.
The winners of the competition earned a place in the third qualifying round of the UEFA Europa League.
Košice were the defending champions.
The games were played on 20/21 October 2009.