Clevedon Town and Rugby Town were the only clubs relegated this season, while Merthyr Tydfil were expelled from the league after failing to meet a FA deadline to give assurances that they could trade effectively during 2010–11, liquidated and reborn under the name Merthyr Town three divisions below Southern League Premier Division.
Bury Town 8 – 2 Barton Rovers, 8 December 2009 Romulus 7 – 1 Beaconsfield SYCOB, 13 February 2009
Division One South & West consisted of 22 clubs, including 16 clubs from previous season and six new clubs: Windsor & Eton won the division and got a place in Premier Division along with play-off winners Cirencester Town, who returned there at the second push.
Hayes finished second bottom but were reprieved from relegation due to higher league clubs problems.
Thus, Bracknell Town, finished bottom of the table, were the only club relegated to the lower league this season.