The 2009–10 Toto Cup Al was the twenty-eighth season of the third most important football tournament in Israel since its introduction and sixth under the current format.

First, sixteen Premier League teams were divided into four groups.

The winners and runners-up, were advanced to the knockout phase.

The defending champions were Maccabi Tel Aviv, making it their third Toto Cup title overall.

The draw for the semi-finals took place on 31 December 2009, with matches played two weeks later on 13 January 2010.