The UEFA Women's Champions League 2009–10 was the first edition of the newly branded tournament, and the ninth edition of a UEFA tournament for women's champion football clubs.

For the first time the top 8 leagues of the UEFA were awarded two entry places in this year's season.

Germany even got 3 entries, as FCR 2001 Duisburg finished outside the top 2 in Germany's league but gained entry as the title holder.

[25] Teams marked (H) hosted a mini-league.

Matches were played at Matija Gubec Stadium, Krško and Ivančna Gorica Stadium, Ivančna Gorica.

Matches were played at Gradski vrt, Osijek and Stadion Cibalia, Vinkovci.

The seeded team played the second leg at home.

[26] Matches were played on 30 September and 7 October.

From this round onwards, there was no seeding, and clubs from the same association could be drawn against each other.

Therefore, instead of drawing specific teams matches were drawn with the winners playing each other in this round.

The top goal scorers including qualifying rounds were: