The 2009–10 season was the 19th season of competitive association football in Ukraine since dissolution of the Soviet Union.

At the meeting of the Professional Football League of Ukraine after the season, it was confirmed that Ukrainian First League team FC Desna Chernihiv failed attestation and hence would have their license withdrawn.

To allow an extra team to be promoted, the PFL determined that a playoff game between the 2nd placed teams from Druha Liha – Kremin Kremenchuk and Nyva Vinnytsia would determine the vacancy created.

This playoff game was played June 28, 2010.

Note: For all scratched clubs, see section Clubs removed for more details 2010 Vyshcha Liha (women)