The 2009 Cal Poly Mustangs football team represented California Polytechnic State University, San Luis Obispo as member of the Great West Conference (GWC) during the 2009 NCAA Division I FCS football season.
Led by first-year head coach Tim Walsh, Cal Poly compiled an overall record of 4–7 with a mark of 1–3 in conference play, placing last out of five teams in the GWC.
The Mustangs played home games at Alex G. Spanos Stadium in San Luis Obispo, California.
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