The Women's time trial of the 2009 Dutch National Time Trial Championships cycling event took place on 27 August 2009 in and around Zaltbommel, Netherlands.

The competition was run over a 22.3 km flat course and the first rider started at 17:10.

[1] Riders started 1 minute apart from each other.

35 cyclists participated in the contest.

[2] Regina Bruins won the time trial in a time of 29' 06.55", ahead of Kirsten Wild and Ellen van Dijk.