The 2009 Varsity Cup was contested from 2 February to 30 March 2009.
The tournament (also known as the FNB Varsity Cup presented by Steinhoff International for sponsorship reasons) was the second season of the Varsity Cup, an annual inter-university rugby union competition featuring eight South African universities.
The tournament was won by Maties for the second consecutive season; they beat NWU Pukke 11-6 in the final played on 30 March 2009.
The winners of these semi-finals will play each other in the final, at the home venue of the higher-placed team.
The following teams took part in the 2009 Varsity Cup competition: