The 2010 Copa Petrobras Asunción was a professional tennis tournament played on clay courts.

It was the fifth edition of the tournament which is part of the 2010 ATP Challenger Tour.

It took place in Asunción, Paraguay between 12 and 17 October 2010.

The following players received wildcards into the singles main draw: The following players received entry from the qualifying draw: Rui Machado def.

Ramón Delgado, 6–2, 3–6, 7–5 Fabio Fognini / Paolo Lorenzi def.