The four group winners had the opportunity to promote directly and become the overall Segunda División B champion.
The six winners advanced with the two losing semifinalists to determine the four teams that entered the last two-legged series for the last two promotion spots.
The draw were held in the RFEF headquarters, in Las Rozas (Madrid), on 10 May 2010, 16:30 CEST.
The aggregate winners will be promoted and qualified to the 2010–11 Segunda División B Final.
The aggregate losers will be relegated to the Non-champions promotion play-off Second Round.