Etzella Ettelbruck and Jeunesse Canach were relegated to the Division of Honour after finishing 13th and 14th in the previous season.
Jeunesse Canach were relegated after one year in the top flight while Etzella leave after an eight-year stay in the league.
Kayl-Tétange are taking part in this competition for the first time in their history, while Rumelange return to the league after a one-year absence.
Wiltz 71 as 12th-placed team had to compete in a single play-off match against 3rd-placed Division of Honour side Hostert.
Hostert won the match after a penalty shootout, thus winning promotion to the league for the first time in their history.