Shakhtar Donetsk were the defending Ukrainian Cup champions and as a member of the Premier League enter the competition in the Round of 32.

[4] ^(1) Makiyivvuhillya Makiyivka were drawn to play away against Bastion Illichivsk but they withdrew from the professional ranks prior to the start of the 2011–12 season.

^(2) Slovkhlib Slovyansk were drawn to play at home against Zhytychi Zhytomyr but they failed attestation and were not admitted to the PFL.

^(3) Desna Chernihiv were drawn to play at home against Nyva Ternopil but they withdrew from the Cup competition having not completed attestation awaiting their status in the PFL.

Nyva Vinnytsia informed the PFL that the club was having financial difficulties and would not arrive for the scheduled cup game against Tytan Armyansk.

The draw took place September 8, 2011[7] and was performed by Anatoliy Kon'kov who was invited as a guest by the Premier League.

Note: ^(6) Arsenal Kyiv had been using Lobanovskyi Dynamo Stadium as a home ground during the season moved their cup game to Obolon Arena.

The draw took place October 13, 2011[10] and was performed by the former Metalist Kharkiv player Ihor Yakubovskiy, who was invited as a guest by the Premier League.

The draw took place March 15, 2012 [12] and was performed by former Dynamo and Ukraine national team coach Yozhef Sabo.