Appearing in this round were bottom 9 teams from 2011–12 Serbian First League, as well as 5 regional cup winners.
Bottom 9 teams from 2011–12 Serbian First League (RFK Novi Sad, OFK Mladenovac, Kolubara, Čukarički, Banat, Radnički Sombor, Mladi Radnik, Sinđelić Niš and Srem) were set as seeded teams, with 5 regional cup winners (Dunav, Kovačevac, Timok, Jedinstvo Užice and Trepča) being set as unseeded teams.
16 teams from 2011–12 Serbian SuperLiga (Partizan, Red Star, Vojvodina, Jagodina, Sloboda Užice, Radnički 1923, Spartak Subotica, OFK Beograd, Javor, Rad, Hajduk Kula, BSK Borča, Smederevo, Novi Pazar, Borac Čačak (II) and Metalac (II)) were set as seeded teams.
[2] No extra time was played if the score was tied after regular 90 minutes, with games going straight into penalties.
[3] Seedings were determined by last seasons final standings in top two Serbian divisions.
Seeded teams: Partizan, Red Star, Vojvodina, Jagodina, Spartak Subotica, OFK Beograd, Javor and Rad.
Seeded teams: Red Star, Borac Čačak (II), Vojvodina and Jagodina.
4 winners from Quarter finals (Vojvodina, Jagodina, OFK Beograd and Javor) took part in this stage of the competition.