She struggled to escape for much of the 129-kilometre eight-lap race, but finally got clear on the intimidating Cauberg Hill to sprint to victory on the last two ascents.
With a finishing time of three hours 14 minutes and 29 seconds, Vos defeated Rachel Neylan of Australia by 10 seconds, with Italy's Elisa Longo Borghini finishing third.
The circuit included the Bemelerberg, 900 m long and maximal 7% and the Cauberg hill in Valkenburg – where the Amstel Gold Race has finished since 2003, and hosted the finish of stage 3 of the 2006 Tour de France – a 1.5 kilometres (0.93 mi) long climb with a maximum gradient of 12%.
The finish was 1.7 kilometres (1.1 mi) beyond the summit of the Cauberg hill.
[1] Of the race's 132 entrants, 80 riders completed the full distance of 129 km (80.2 mi).