AC Horsens and Silkeborg IF finished the 2012–13 season of the Superliga in 11th and 12th place, respectively, and were relegated to the 1st Division.
They replaced Viborg FF and FC Vestsjælland, who were promoted to the 2013–14 Danish Superliga.
Hvidovre IF and BK Marienlyst won promotion from the 2012–13 Danish 2nd Divisions.
As of 1 July 2013 FC Hjørring will participate under the name Vendsyssel FF.
Note: Flags indicate national team as has been defined under FIFA eligibility rules.