The 2013–14 Amlin Challenge Cup pool stage will be the opening stage of the 18th season of the European Challenge Cup, the second-tier competition for European rugby union clubs.

The quarter-finalists will then participate in a knockout tournament that ultimately ends with the final at the Arms Park in Cardiff on Friday 23 May 2014.

[2] The draw for the pool stage took place on 5 June 2013 at the Aviva Stadium in Dublin.

[3] The dates and times of the first 4 rounds were announced on 27 July 2013.

[4] Lusitanos XV replaced Olympus Madrid on 2 September 2013, as the latter withdrew due to financial issues.