A total of 22 teams contested the league: 15 of which were returning from the 2012–13 season, 4 of which were promoted from Lega Pro Prima Divisione, and three relegated from Serie A.
Four teams were promoted from Lega Pro Prima Divisione, with only two certain as of May 2013: Avellino, after four seasons, and Trapani, which will make their debut in the league.
On 16 June, the winners of the Lega Pro Prima Divisione play-off finals were determined as two newcomers: Carpi and Latina.
When rumors about Siena's probable collapse arrived to the FIGC in May, the Federation decided to seize the opportunity to reduce the league.
When the CONI agreed with the Piedmontese club, the angry FIGC decided to choose a Lega Pro team instead of Novara.