The Women's team time trial of the 2013 UCI Road World Championships took place on 22 September 2013 in the region of Tuscany, Italy.

The course of the race was 42.79 km (26.6 mi) from the town of Pistoia to the Nelson Mandela Forum in Florence.

[5] Specialized–lululemon, which won the team time trial at the 2012 Championships, was the favourite for the event.

The team won the 42.5 km (26.4 mi) UCI World Cup team time trial race, the Open de Suède Vårgårda TTT 38 seconds ahead of Rabobank–Liv Giant, who finished fourth at the 2012 championships, and 1' 26" ahead of 2012 runners-up Orica–AIS on 16 August.

As well as this, Specialized–lululemon won all team time trials held during the 2013 season; at the Lotto–Belisol Belgium Tour, they won the 19.75 km (12.3 mi) team time trial by 35 seconds from Orica–AIS, while Rabobank–Liv Giant did not participate.