The Women's time trial of the 2013 UCI Road World Championships took place on 24 September 2013 in the region of Tuscany, Italy.

The course of the race was 22.05 km from Parco delle Cascine to the Nelson Mandela Forum in Florence.

[1][2] Ellen van Dijk from the Netherlands lived up her expectations as main favourite and won the time trial by dominating the race, beating perennial podium finisher Linda Villumsen and the surprising American Carmen Small.

Van Dijk did not win two time trials, Emma Johansson from Sweden beat her in the Emakumeen Euskal Bira and Shara Gillow from Australia in the Thüringen Rundfahrt der Frauen.

Van Dijk lost one second to Villumsen in the final five kilometres but her margin was large enough to win her second world title of the championship after winning the team time trial with her Specialized–lululemon squad on Sunday.

Van Dijk lived up the expectations as main favourite and won the time trial by dominating the race.

I maybe got over excited and a went too fast, but I maintained the time difference and so it was all ok."[6] This race was the seasons' main goal for Van Dijk.

[7] Van Dijk told she practiced the course twice in August at 5:00am to avoid traffic and made video recordings of the course to get to know the turns.

[8] Villumsen rode the New Zealand National Time Trial Championships in January before backing off until June.

Ellen van Dijk has been riding very well all throughout the year, she has won all kinds of time trials, short ones, long ones.

"[6][9] Small, American national time trial champions, considered retirement in 2012 before signing by Specialized–lululemon.

With 100 meters to go I wanted to quit, but I did one more click down on my gears and I just counted the pedal strokes to get me to the finish.