The girls' 100 metres hurdles at the 2013 World Youth Championships in Athletics was held from 10 to 11 July.

Prior to the competition, the following records were as follows.

Qualification rule: first 3 of each heat (Q) plus the 6 fastest times (q) qualified.

[1] Qualification rule: first 2 of each heat (Q) plus the 2 fastest times (q) qualified.

[2] [3]