Six teams entered the LNFA Serie A, the top-tier level of American football in Spain.
On December 28, the three teams from the Community of Madrid (Rivas Osos from Serie A, Camioneros Coslada and Las Rozas Black Demons from Serie B) were excluded from the competition as they did not have a junior team in the junior league.
L'Hospitalet Pioners and Mallorca Voltors were relegated from previous Serie A season.
The three teams from the Community of Madrid that were excluded in December 2015 from the 2015–16 season took part in the promotion playoffs.
The three teams from the Community of Madrid that were excluded in December 2015 from the 2015–16 season will take part in the promotion playoffs.