The Ospreys were led by seventh-year head coach Matthew Driscoll and played their home games at UNF Arena on the university's campus in Jacksonville, Florida as members of the Atlantic Sun Conference (A-Sun).

[1] As the No.1 seed in the Atlantic Sun tournament, they advanced to the semifinals where they were upset at home by Florida Gulf Coast.

The Ospreys made the most three point shots (402) out of all NCAA Division I teams in the 2015–16 season, earning them the nickname "Birds of Trey".

[6] Roster Last update: November 26, 2015 The Ospreys carried their previous season's momentum right into their first game by earning a road upset of Illinois by a score of 93–81.

Guard Dallas Moore recorded his second straight double-double and became UNF's 11th player to score 1,000 career points.

The Ospreys led 56–48 at halftime, but 43 points by freshman phenom Ben Simmons proved too much for UNF to handle.

[15] In December, North Florida swept a four-game home stand to extend their winning streak at UNF Arena to 14 games, a program record.

[17] On January 9, the Ospreys defeated crosstown rival Jacksonville for the fifth time in a row in the River City Rumble.

On February 20, the Ospreys clinched at least a share of the Atlantic Sun regular season title by defeating USC Upstate, 81–78.

The Ospreys began their quest to win back-to-back Atlantic Sun Tournament titles by defeating USC Upstate in the quarterfinals, 92–69.

Due to renovations at the O'Connell Center, the 7-seed Ospreys hosted the 2-seed Florida Gators in the first round of the 2016 National Invitation Tournament, where they fell 97–68 in front of 6,011 fans at the UNF Arena.