The champion would win the trophy, a cash prize of €90,152 and the qualification for the next year tournament.
[1] The competition began in late July 2016 with the first games of the Asturias, Castile-La Mancha and Extremadura tournaments and finished on 6 April 2017 with the final of national phase.
Atlético Saguntino achieved its first national title ever after defeating Fuenlabrada in the finals.
[4] The champion would get a prize of €3,000 and the 50% of the income in the final game, played in a neutral venue.
[7] On 9 September 2016, the Castile-León Football Federation confirmed that three teams will join the tournament.
[10] Albacete, Conquense, Socuéllamos and Toledo are ineligible to qualify to the national stage as regional champions, as they participate in the Copa del Rey.
With a record of participants in the Regional competition, the games of the first round, drawn on 1 July 2016, will be played at the pitch of the worst qualified team in the 2015–16 season.
Jerez received a bye for the fourth round while the winner between Amanacer and Cacereño would qualify directly for the final.
Andorra (4) Atlético Mancha Real (3) Atlético Saguntino (3) Boiro (3) Burgos (3) Conquense (4) Laredo (4) Lorca Deportiva (4) Murcia (3) Ponferradina (3) Prat (3) Real Unión (3) San Sebastián de los Reyes (3) Socuéllamos (3) UD Logroñés (3) Villa de Santa Brígida (4) Zamora (4) Zamudio (3)
Alavés B (4) Arandina (3) Arosa (4) Badajoz (4) Badalona (3) Fuenlabrada (3) Linares (3) Madridejos (4) Orihuela (4) Peña Sport (4) Poblense (4) Racing Santander B (4) San Fernando (3) Sporting Gijón B (4) Tenisca (4) Teruel (4) UCAM Murcia B (4) Varea (4)