63 teams contested, beginning on 6 September 2016 with the first of six rounds, and ended on 30 April 2017 with the final played at De Kuip in Rotterdam.
This edition was notable for several unicums, which included holding the first test in an official match of a top tier-domestic competition of the Video assistant referee-system.
[1] Anouar Kali held the distinction of becoming the first player to receive a red card after an intervention from the video referee in the first round-game between Ajax and his team Willem II.
They also participated in the 2017 edition of the Johan Cruyff Shield, the Dutch Supercup match at the start of next season between the Cup winner and the champions of the Eredivisie, Feyenoord.
The lowest ranked team left were FC Volendam and SC Cambuur from the second tier of Dutch football.