2016–17 Lowland Football League

Threave Rovers declined the opportunity to re-apply for membership following their bottom place finish the previous season and instead rejoined the South of Scotland Football League.

[2] Hawick Royal Albert and Civil Service Strollers joined the league from the East of Scotland Football League to bring the number of clubs to 16 for the first time.

The league was won with two matches still to play by East Kilbride on 1 April 2017 after they defeated Gala Fairydean Rovers 6–1.

East Kilbride defeated the winners of the 2016–17 Highland Football League (Buckie Thistle) in the League Two play-offs semi-finals, but lost to Cowdenbeath on penalties in the final.

However Lothian Thistle Hutchison Vale did not meet the required licensing criteria therefore Edusport Academy were promoted automatically to replace Preston Athletic.