The 2016–17 Melbourne City FC W-League season was the club's second season in the W-League, the premier competition for women's football in Australia.

The team is based at the City Football Academy at La Trobe University and plays home games at both AAMI Park and CB Smith Reserve.

The club was again by coached by manager Joe Montemurro,[1] though in January 2017 Montemurro was promoted to assistant manager of the senior men's team and his role as manager was replaced by playing captain Jess Fishlock, who acted as captain/coach for the remainder of the season.

[3] Melbourne City finished fourth in the league, qualifying for the finals series.

[4] Note: Flags indicate national team as defined under FIFA eligibility rules; some limited exceptions apply.