[1] This round featured the bottom 5 teams from the 2015–16 Serbian First League, as well as 5 regional cup winners.
Furthermore, Sloga PM, which earned a place in the first round via last season's final position in the First League, was also dissolved.
Thus, both teams from the proposed tie Radan Lebane - Dinamo Vranje received an automatic bye to the first round.
Drawing of the lots was conducted by former national team player and head coach Vladimir Petrović.
[3] Teams that played below Serbian First League in the previous season (Žarkovo, Radan Lebane, Polet Ljubić and Mladost Bački Jarak) were automatically named as hosts.
[4] The seeds were determined by last season's final standings in the Serbian top divisions.
Seeded teams: Red Star Belgrade, Partizan, Čukarički, Vojvodina, Borac Čačak, Voždovac, Radnik Surdulica and Mladost Lučani.
Drawing of the lots was conducted by the current head coach of U21 national team, Tomislav Sivić.
Seeded teams: Partizan, Borac Čačak, Red Star Belgrade and Čukarički.
Unseeded teams: Vojvodina, Voždovac, Mladost Lučani and Sloboda Užice (II).
The 4 winners from the Quarter finals (Partizan, Red Star Belgrade, Čukarički and Vojvodina) took part in the semi-finals.