The 2016 AFC Champions League knockout stage was played from 17 May to 26 November 2016.
The away goals rule, extra time (away goals do not apply in extra time) and penalty shoot-out were used to decide the winner if necessary (Regulations Article 12.3).
In the quarter-finals, the four teams from the West Zone were drawn into two ties, and the four teams from the East Zone were drawn into the other two ties, with the order of legs also decided by the draw.
[4] The draw for the quarter-finals was held on 9 June 2016, 16:00 MYT (UTC+8), at the Petaling Jaya Hilton Hotel in Kuala Lumpur, Malaysia.
[5] There was no seeding or country protection, so teams from the same association could be drawn into the same tie.
The El Jaish v Al-Nasr first leg, originally won 3–0 by Al-Nasr, was forfeited and awarded 3–0 to El Jaish by the AFC Disciplinary Committee on 12 September 2016, as Al-Nasr fielded the player Wanderley, who was found to be registered using a fake Indonesian passport.