The 2016 Aegon Eastbourne Trophy was a professional tennis tournament played on outdoor grass courts.

It was the second edition of the tournament and part of the 2016 ITF Women's Circuit, offering a total of $50,000 in prize money.

It took place in Eastbourne, United Kingdom, on 30 May–5 June 2016.

The following player received a wildcard into the singles main draw: The following players received entry from the qualifying draw: The following player received entry by a lucky loser spot: The following players received entry by protected rankings: