The 2016 Currie Cup First Division was the second tier of the second stage of the 2016 Currie Cup, the 78th edition of this annual South African rugby union competition organised by the South African Rugby Union.
It was played between 12 August and 7 October 2016 and featured six teams that qualified through the 2016 Currie Cup qualification competition.
The tournament was won by the Griffons for the third time in their history; they beat the Leopards 44–25 in the final played on 7 October 2016.
The remaining six teams from the qualification stage qualified for the Currie Cup First Division.
For each round, each team's cumulative points total is shown with the overall log position in brackets.