The Tigers played their home games at Faurot Field in Columbia, Missouri, and competed in the Eastern Division of the Southeastern Conference (SEC).

Due to National Collegiate Athletic Association (NCAA) action, all of Missouri's wins for the 2015 and 2016 seasons were vacated.

The 2016 schedule consisted of 7 home and 5 away games in the regular season.

The Tigers hosted SEC foes Arkansas, Georgia, Kentucky, and Vanderbilt, and travelled to Florida, LSU, South Carolina, and Tennessee.

The Tigers played the West Virginia Mountaineers from the Big 12 Conference as an away game.