Coached by Anthony Griffin and captained by Matt Moylan,[3] the Panthers competed in the NRL's 2016 Telstra Premiership.

The Panthers started their season with a string of close results, but failed to win consecutive games during the competition's opening two months.

Over the course of the year, new coach Anthony Griffin redefined who was already well established within the team,[4][5] and experienced playmakers Jamie Soward and James Segeyaro would later be released from their contracts mid-season.

1 Parramatta were deducted 12 competition points and their for/against tally accumulated between rounds 1-9 for gross long-term salary cap breaches.

In addition to competing in the National Rugby League, the Panthers also fielded semi-professional teams in the National Youth Competition's 2016 Holden Cup (for players aged under 20) and the New South Wales Rugby League's 2016 Intrust Super Premiership (NSW Cup).