Carla Suárez Navarro defeated Jeļena Ostapenko in the final, 1–6, 6–4, 6–4 to win the singles tennis title at the 2016 WTA Qatar Open.

Lucie Šafářová was the defending champion, but lost to Çağla Büyükakçay in the second round.

The top eight seeds received a bye into the second round.

Click on the seed number of a player to go to their draw section.