Simona Halep defeated Madison Keys in the final, 7–6(7–2), 6–3 to win the women's singles tennis title at the 2016 Canadian Open.

Belinda Bencic was the reigning champion, but withdrew with a left wrist injury before the tournament.

The top eight seeds received a bye into the second round.

Click on the seed number of a player to go to their draw section.