All teams in the Scottish Women's Football League and SWPL 1 & 2 were eligible to enter.
Edinburgh Caledonia & Dumbarton United received a bye to the second round.
The final was played between Glasgow City and Hibernian on 6 November 2016 at New Douglas Park in Hamilton.
It featured the same teams as the previous year's final and the SWPL Cup final earlier in the year (as well as the previous two editions of that competition).
[1][6][7] Hibs men's team had also won the 2015–16 Scottish Cup, and this was the first instance of a club holding both national trophies at the same time (albeit the women's team was only an affiliate and not a fully integrated element of the club at that point, and due to the differing schedules this was only for six months rather than a whole year).