The 2016 Southern Miss Golden Eagles football team represented the University of Southern Mississippi in the 2016 NCAA Division I FBS football season.

The Golden Eagles played their home games at the M. M. Roberts Stadium in Hattiesburg, Mississippi, and competed in the West Division of Conference USA (C–USA).

They were led by first-year head coach Jay Hopson.

Southern Miss announced its 2016 football schedule on February 4, 2016.

The Golden Eagles will host C–USA foes Charlotte, Marshall, Louisiana Tech, and Rice, and will travel to North Texas, Old Dominion, UTEP, and UTSA.