The 2016 Southland Conference tournament was held at Cowgirl Diamond on the campus of McNeese State University in Lake Charles, Louisiana, from May 11 through 13, 2016.
The Championship game was broadcast on ESPN3 with the remainder of the tournament airing on the Southland Digital Network.
Abilene Christian and Incarnate Word were ineligible due to their transition from D2 to D1.
Since Abilene Christian was ineligible for tournament play, the fourth through seventh place finishers moved up in seeding.
In addition, the Sam Houston Bearkats, finishing the regular season in seventh place, qualified for the tournament.