The 2016 Villanova Wildcats football team represented Villanova University in the 2016 NCAA Division I FCS football season.

They were led by 32nd-year head coach Andy Talley and played their home games at Villanova Stadium.

They were a member of the Colonial Athletic Association.

They finished the season 9–4, 6–2 in CAA play to finish in a tie for second place.

They received an at-large bid to the FCS Playoffs where they defeated St. Francis (PA) in the first round before losing in the second round to South Dakota State.