The defending champions were Viktoria Plzeň, who won their fifth Czech title the previous season.
[2] Dukla Prague lost seven consecutive games at the start of the season, becoming the first team in the Czech First League to do so.
Teams placed between 7th and 10th position will take part in the Europa league play-offs.
MFK Karviná won 3–2 on aggregate and retained their spot in the 2019–20 Czech First League; Vysočina Jihlava remained in the 2019–20 Czech National Football League.
FK Příbram won 3–3 on aggregate with away goals and retained their spot in the 2019–20 Czech First League; FC Zbrojovka Brno remained in the 2019–20 Czech National Football League.